HSP27 (HSPB1) Mouse Monoclonal Antibody [Clone ID: G3.1]

CAT#: AM33370PU-T

HSP27 (HSPB1) mouse monoclonal antibody, clone G3.1, Purified

Product Images

Formalin-Paraffin breast carcinoma (10X) stained with HSP27 Antibody (Clone G3.1).

• 

  Formalin-Paraffin breast carcinoma (20X) stained with HSP27 Antibody (Clone G3.1).

Specifications

Product Data

• Clone Name: G3.1
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use Antibody without BSA for Coating.
  Flow Cytometry: 0.5-1 μg/10⁶ cells.
  Immunofluorescence: 0.5-1 μg/ml.
  Western Blot: 0.5-1 μg/ml.
  Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: ~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor.
• Reactivities: Chimpanzee, Chicken, Human, Monkey, Mouse, Rat, Sheep
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
• Specificity: It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced “p29” and “24K” protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
  Cellular Localization: Predominantly cytoplasmic with some nuclear.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 27 kDa
• Database Link:
  Entrez Gene 3315 Human
  UniProt ID P04792
• Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein, SRP27, HSP25