c-Myc (MYC) Mouse Monoclonal Antibody [Clone ID: CT14.G4]

CAT#: AM33356PU-S

c-Myc (MYC) mouse monoclonal antibody, clone CT14.G4, Purified

Specifications

Product Data

• Clone Name: CT14.G4
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 μg/million cells.
  Immunofluorescence: 1-2 μg/ml.
  Western Blotting: 0.5-1 μg/ml.
  Immunoprecipitation: 1-2 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
  Positive Control: HL-60 cells or breast carcinoma.
• Reactivities: Chimpanzee, Gorilla, Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A synthetic peptide, corresponding to aa 408-439 from C-terminus of Human c-myc, coupled to KLH.
• Specificity: Recognizes c-Myc Oncoprotein.
  Cellular Localization: Nuclear.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 62-64 kDa
• Gene Name: v-myc avian myelocytomatosis viral oncogene homolog
• Database Link:
  Entrez Gene 4609 Human
  UniProt ID P01106
• Background: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.
• Synonyms: Transcription factor p64, BHLHE39