c-Myc (MYC) Mouse Monoclonal Antibody [Clone ID: MYC699]

CAT#: AM33353PU-S

c-Myc (MYC) mouse monoclonal antibody, clone MYC699, Purified

Specifications

Product Data

• Clone Name: MYC699
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 μg/million cells.
  Immunofluorescence: 1-2 μg/ml.
  Western Blotting: 0.5-1 μg/ml.
  Immunoprecipitation: 1-2 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
  Positive Control: HL-60 cells or breast carcinoma.
• Reactivities: Chimpanzee, Gorilla, Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A synthetic peptide, corresponding to aa 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQL-RNSCA) from C-terminus of Human c-myc, coupled to KLH.
• Specificity: Recognizes c-Myc Oncoprotein.
  Cellular Localization: Nuclear.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 62-64 kDa
• Database Link:
  Entrez Gene 4609 Human
  UniProt ID P01106
• Background: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiatio n, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/ Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase ( JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c -Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribut e blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.
• Synonyms: Transcription factor p64, BHLHE39