TRP1 (TYRP1) Mouse Monoclonal Antibody [Clone ID: TA99]

CAT#: AM33299PU-T

TRP1 (TYRP1) mouse monoclonal antibody, clone TA99, Purified

Specifications

Product Data

• Clone Name: TA99
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 µg/million cells.
  Immunofluorescence: 1-2 µg/ml.
  Western Blotting (Not tested): 0.5-1 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Positive Control: SK-MEL-23, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: SK-MEL-23 cells.
• Specificity: It reacts with a 75kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1).
  Cellular Localization: Cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 75 kDa
• Gene Name: tyrosinase related protein 1
• Database Link:
  Entrez Gene 7306 Human
  UniProt ID P17643
• Background: Tyrosinase-related protein-1 (TRP-1) is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi. Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.
  Defects in TRP-1 affect pigmentation in melanosomes, and have been shown to cause two diseases dependent on skin colour; rufous oculocutaneous albinism (ROCA) occurs predominantly in southern African blacks and oculocutaneous albinism type III (OCA-III/3) a form of albinism with only moderate reduction of pigment.
• Synonyms: Tyrosinase-related protein 1, TYRP, TYRRP, TRP-1, TRP1, DHICA oxidase, Catalase B, CAS2