Biotin Mouse Monoclonal Antibody [Clone ID: SPM375]

CAT#: AM33281PU-S

Biotin mouse monoclonal antibody, clone SPM375, Purified


USD 504.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name SPM375
Applications ELISA, FC, IF, IHC, ISH, WB
Recommended Dilution ELISA. 
Western Blotting.
Confocal Microscopy. 
Flow Cytometry: 0.5-1 µg/106 cells. 
In situ Nucleic Acid Hybridization. 
Immunofluorescence: 0.5-1 µg/ml. 
Immunohistochemistry on Frozen and Formalin-Fixed 
Sections: 0.5-1 µg/ml for 30 minutes at RT. 
No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Recommended Positive Control: Biotinylated proteins in solution or on tissues.
Reactivities All Species
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Biotinylated Sheep immunoglobulin.
Specificity It recognizes both the free and protein-conjugated (either soluble or cell bound) form of Biotin. 
This Monoclonal Antibody is highly specific to Biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for Biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-Biotin antibody may be substituted to decrease this noise.
Cellular LocalizationDepends on the target.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Stability Shelf life: one year from despatch.
Predicted Protein Size 244 kDa
Synonyms Vitamin B7, Vitamin H
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.