Phosphotyrosine Mouse Monoclonal Antibody [Clone ID: SPM102]

CAT#: AM33265PU-S

Phosphotyrosine mouse monoclonal antibody, clone SPM102, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded breast carcinoma stained withPhosphotyrosine Antibody Cat.-No AM33265PU (Clone SPM102). Note cell surface and cytoplasmic staining.

Specifications

Product Data

• Clone Name: SPM102
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for Coating.
  Western Blot: 0.5-1 µg/ml.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 1-2 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 1-2 µg/ml for 30 minutes at RT.
  No special pretreatment is required for staining of formalin/paraffin tissues.
  Recommended Positive Control: MCF-7, MDA-231, T47-D cells or breast carcinoma.
• Reactivities: All Species
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Phosphotyrosine conjugated to KLH
• Specificity: Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins.
  This Monoclonal antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
  Cellular Localization: Depends upon the location of phosphorylated target.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: Depends upon the phosphorylated target
• Background: Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation.