ACTC1 Mouse Monoclonal Antibody [Clone ID: 22D3]

SKU AM33001PU-N

ACTC1 mouse monoclonal antibody, clone 22D3, Purified

Product Data

• Clone Name: 22D3
• Application: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA. ; Flow Cytometry. ; Immunoblotting.; Immunofluorescence.; Immunohistochemistry on Frozen Sections.; Immunohistochemistry on Paraffin-Embedded Sections. ; Recommended Dilutions: 1/100-1/1000 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/1000-1/5000 for immunoblotting applications.
• Reactivity: Human, Porcine, Rabbit, Rat, Zebrafish
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide comprising the N-terminal nonapeptide of α-Cardiac Actin with an acetylated N-terminus coupled to keyhole limpet hemocyanin through the cysteine residue.
• Specificity: This 22D3 Monoclonal antibody is highly specific for Alpha-Cardiac Actin, and does not cross react with other Actin isoforms.
• Buffer: PBS; State: Purified; State: Liquid purified IgG fraction; Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freeze-thaw cycles.
• Stability: Shelf life: One year from despatch.
• Shipping: Blue Ice
• Gene Name: actin, alpha, cardiac muscle 1
• Database Link:
  P68032
• Background: Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates. ; Cardiac α-actin, which is the main actin isoform in the adult heart, has also been shown to be the predominant form in early muscle development. In later development the expression of α-cardiac actin is down regulated and α-skeletal actin becomes the dominant isoform in the adult skeletal muscle.
• Synonyms: ACTC; ASD5; CMD1R; CMH11

Reference Data