CEACAM16 Mouse Monoclonal Antibody [Clone ID: SU-9D5]

CAT#: AM32984PU-N

CEACAM16 mouse monoclonal antibody, clone SU-9D5, Purified

Product Images

SDS-PAGE analysis of purified SU-9D5 monoclonal antibody: Lane 1: molecular weight marker, Lane 2: 2 ug of purified SU-9D5 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.

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  Immunohistological staining using SU-9D5 on paraffin sections of cochlea tissue of an adult transgenic mouse with a human CEACAM16 gene (Ceacam16+/+ mouse cochlea). Binding of biotinylated SU-9D5 was detected with horseradish peroxidase-coupled streptavidin and stained with 3-amino-9-ethylcarbazole.

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  BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3, 4, 6, 8 19, or 20. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (data not shown). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified SU-9D5 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM16 transfected cells (red curve).

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  FACS analysis of BOSC23 cells using SU-9D5. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM16 (red curve) or an irrelevant protein (control transfectant). Binding of SU-9D5 was detected with a PE- conjugated secondary antibody. A positive signal was obtained only with CEACAM16 transfected cells.

Specifications

Product Data

• Clone Name: SU-9D5
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: Flow Cytometry: 1.2 µg/10⁶ cells.
  Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
  Immunohistochemistry on Paraffin Sections: 4 μg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: cDNA encoding Human CEACAM16
• Specificity: Recognizes Human CEACAM16.
  Selection: based on recognition of the complete native protein expressed on transfected mammalian cells.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: carcinoembryonic antigen related cell adhesion molecule 16
• Database Link:
  Entrez Gene 388551 Human
  UniProt ID Q2WEN9
• Background: CEA-related cell adhesion molecule 16 (CEACAM16) belongs to the carcinoembryonic antigen (CEA) gene family which are widely expressed (1). The mammal-specific member CEACAM16 is well conserved and specifically expressed in the inner ear (2). It is a structural component of the tectorial membrane and the expression is necessary for hearing over an extended frequency range (3).
• Synonyms: CEAL2