CD5 Mouse Monoclonal Antibody [Clone ID: C5/473]

CAT#: AM32836PU-T

CD5 mouse monoclonal antibody, clone C5/473, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD5 Antibody (Clone C5/473).

Specifications

Product Data

• Clone Name: C5/473
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Western Blotting: 0.5-1 µg/ml.
  Immunofluorescence: 0.5-1 µg/ml.
  Immunoprecipitation: 5-10 µl/500 µg protein lysate.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: 293T, Ramos, or MOLT-4 Cells, Tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human CD5 recombinant protein.
• Specificity: Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.
  Cellular Localization: Cell surface.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 67 kDa
• Gene Name: CD5 molecule
• Database Link:
  Entrez Gene 921 Human
  UniProt ID P06127
• Background: CD5, a 67KDa Type I transmembrane glycoprotein, is a member of SRCR superfamily expressed specifically of thymocytes, mature T-cells and B-1a cells. CD5 has an extracellular domain with 3 SRCR domains and cytoplasmic tail is devoid of any intrinsic enzyme activity. CD5 functions as a dual receptor modulating either positive or negative co-stimulatory signals depending on the cell type and the developmental stage. It binds CD72 and provides co-stimulatory signal for T-cell growth. CD5 plays an important role in thymocyte development and also fine-tunes TCR signaling. It is associated with antigen-specific receptor complex expressed on T-cells and B-1a cells and regulates T and B-cell mediated responses. CD5 signaling involves various kinases including PI3K, Src family tyrosine kinases, CKII, Ras GAP, and members of MAPK.
• Synonyms: CD5, LEU1