DHLAG (CD74) Mouse Monoclonal Antibody [Clone ID: LN2]

CAT#: AM32811PU-S

DHLAG (CD74) mouse monoclonal antibody, clone LN2, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD74 Antibody (Clone LN2).

Specifications

Product Data

• Clone Name: LN2
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: For coating, Use Antibody without BSA.
  Western Blot: 0.5-1 µg/ml.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 1-2 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen and Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Daudi Cells or Raji Cells. Tonsil or lymph node.
• Reactivities: Baboon, Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: SU-DHL-4 lymphoma cells.
• Specificity: This monoclonal LN2 antibody can be used for the identification of B cell lymphoma and leukemias. It also stains activated neoplastic cells in T cell lymphomas and Sternberg-reed cells.
  It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV).
  Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
  Cellular Localization: Cell surface and paranuclear globular.
  Negative Species: Rat.
• Formulation: 10 mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 33-41 kDa
• Gene Name: CD74 molecule
• Database Link:
  Entrez Gene 972 Human
  UniProt ID P04233
• Background: CD74, a Type-II transmembrane protein, is a unique member of RIP-processed protein family. It consists of an extracytoplasmic domain, a transmembrane segment and an intracytoplasmic domain. It is expressed in B-cells, monocytes, macrophages and other Class II MHC expressing cells. CD74 associates with alpha and beta chains of HLA-DR (MHC class II) and directs intracellular sorting of MHC class II molecules. It has a prominent role in antigen presentation and as an accessory signaling molecule. Cell-surface CD74 binds to MIF and its expression is required for MIF-mediated ERK1/2-phosphorylation, PGE2 production and cell proliferation. CD74 regulates B-cell differentiation and acts as a survival receptor through activation of NF-kB p65/RelA homodimer. Pathological role of CD74 has been identified in different cancers including Multiple Myeloma.
• Synonyms: p33, DHLAG

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Antigen processing and presentation