Cd4 Rat Monoclonal Antibody [Clone ID: GK1.5]

CAT#: AM32703FC-N

Cd4 rat monoclonal antibody, clone GK1.5, FITC, Purified

Product images

BALB/c mouse splenocytes were stained with 0.031 ug /106 cells FITC conjugated anti-Mouse CD4 Antibody Cat.-No AM32703FC-N (Clone GK1.5) and cytocentfuged onto a glass slide prior to imaging by fluorescence microscopy (original magnification 100x)

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  BALB/c Mouse splenocytes were stained with a 0.031 ug/106 cells FITC conjugated anti-Mouse CD4 Antibody Cat.-No AM32703FC-N (Clone GK1.5) (purple) or Negative Control (green).

Specifications

Product Data

• Clone Name: GK1.5
• Applications: FC, IF
• Recommended Dilution: Flow Cytometry (Cell Surface): 0.031-1.0 μg/10⁶ cells.
• Reactivity: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Specificity: Recognizes Mouse CD4. Other species not tested.
• Formulation: PBS, pH 7.4
  Label: FITC
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.05% Sodium Azide
  Label: Fluorescein
• Concentration: lot specific
• Purification: Protein G Chromatography
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 52 kDa
• Database Link:
  Entrez Gene 12504 Mouse
  UniProt ID P06332
• Background:

  CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. It is a member of the immunoglobulin superfamily. On T cells, it is the co-receptor for the T cell receptor (TCR) and amplifies the signal generated by the TCR by recruiting the tyrosine kinase lck that is essential for activating many molecules involved in the signaling cascade of an activated T cell. It is an important component of the immune system and is also the cellular receptor for HIV-1. It consists of a cytoplasmic tail, one transmembrane region, and four extracellular domains, D1-D4.

• Synonyms: T-cell surface antigen T4/Leu-3