IL3 Mouse Monoclonal Antibody

CAT#: AM32452PU-N

IL3 mouse monoclonal antibody, Aff - Purified

Product Images

Sandwich ELISA using Interleukin-3 / IL3 Antibody Cat.-No AM32452PU-N

Specifications

Product Data

• Applications: ELISA, Neutralize, WB
• Recommended Dilution:

  Sandwich ELISA: In a Sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 500 pg/ml of recombinant Human IL-3 when used with Biotinylated Antigen Affinity Purified Anti-Human IL-3 (Cat.-No PP1025B) as the detection antibody at a concentration of approximately 0.50-1.0 μg/ml.
  Western Blot: To detect Human IL-3 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-3 is 0.50-1.0 ng/lane, under reducing conditions and 2.0-4.0 ng/lane, under non-reducing conditions.
  Neutralization: To yield one-half maximal inhibition [ND₅₀] of the biological activity of Human IL-3 (1.0 ng/ml), a concentration of 0.007-0.010 μg/ml of this antibody is required.

• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Highly purified E.coli derived Recombinant Human IL-3 (Cat.-No PA076)
• Specificity: This antibody recognizes Human Interleukin-3 / IL3.
• Formulation: PBS without preservatives
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: interleukin 3
• Database Link:
  Entrez Gene 3562 Human
  UniProt ID P08700
• Background:

  IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine.

• Synonyms: IL-3, Multipotential colony-stimulating factor, Hematopoietic growth factor, P-cell-stimulating factor, MCGF