PF4 Mouse Monoclonal Antibody [Clone ID: KKO]

CAT#: AM31851BT-N

PF4 mouse monoclonal antibody, clone KKO, Biotin

Specifications

Product Data

• Clone Name: KKO
• Applications: ELISA, WB
• Recommended Dilution: ELISA: Use at 0.08 μg/ml following coating plate with PF4/ heparin complex. This can be done by incubating PF4 with an excess of heparin (100-200 U/ml), while coating the plate.
  Western blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human Factor IV purified from the supernatant of Thrombin activated platelets.
• Specificity: This antibody is specific for Human Platelet Factor IV/heparin complexes.
  AM31851BT-N (clone KKO), unlike AM31850BT-N (clone RTO) which binds PF4 alone, is specific for PF4/heparin complexes and is seen to have similar binding properties as auto-antibodies found in HIT/HITT.
• Formulation: PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml
  Label: Biotin
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: platelet factor 4
• Database Link:
  Entrez Gene 5196 Human
  UniProt ID P02776
• Background: Platelet Factor IV (PF4) is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high affinity to heparin and plays a role in inflammation and wound repair. PF4 is a chemoattractant for neutrophils, monocytes and fibroblasts and has been reported to be an immunologic regulator that inhibits suppressor T-cell activity.
  
  Heparin-Induced Thrombocytopenia/Thrombosis (HIT/HITT) is a life-threatening complication that manifests itself in a small population of patients exposed to intravenous-heparin. It is characterized by the production of PF4/heparin auto-antibodies.
  These auto-antibodies bind the PF4/heparin complexes and subsequently bind the FCGIIa receptor on the platelets surface through their Fc region. This activates the platelets and can initiate clot formation.
• Synonyms: PF-4, Iroplact, Oncostatin-A, CXCL4, SCYB4

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction