PF4 Mouse Monoclonal Antibody [Clone ID: RTO]

CAT#: AM31850BT-N

PF4 mouse monoclonal antibody, clone RTO, Biotin

Specifications

Product Data

• Clone Name: RTO
• Applications: ELISA, WB
• Recommended Dilution: ELISA: Use at 0.016 μg/ml.
  Western blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human Factor IV purified from the supernatant of Thrombin activated platelets.
• Specificity: This antibody is specific for Human Platelet Factor IV.
  AM31850BT-N (clone RTO) is seen to bind free PF4 in the absence of any bound Heparin. This is unlike AM31851BT-N (clone KKO), which binds PF4/heparin complexes to a similar degree as auto-antibodies found in individuals with Heparin-Induced Thrombocytopenia/Thrombosis (HIT/HITT).
• Formulation: PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
  Label: Biotin
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: platelet factor 4
• Database Link:
  Entrez Gene 5196 Human
  UniProt ID P02776
• Background: Platelet Factor IV (PF4) is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high affinity to heparin and plays a role in inflammation and wound repair. PF4 is a chemoattractant for neutrophils, monocytes and fibroblasts and has been reported to be an immunologic regulator that inhibits suppressor T-cell activity.
• Synonyms: PF-4, Iroplact, Oncostatin-A, CXCL4, SCYB4

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction