p75 NGF Receptor (NGFR) Mouse Monoclonal Antibody [Clone ID: ME20.4]

CAT#: AM31842FC-N

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p75 NGF Receptor (NGFR) mouse monoclonal antibody, clone ME20.4, FITC



USD 785.00


Availability*
2 Weeks

Size
    • 100 ug


Product images

Specifications

Product Data
Clone Name ME20.4
Applications FC, IF, IHC, IP, Neutralize, WB
Recommended Dilution Flow Cytometry.
Reactivity Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen WM245 melanoma cells
Specificity Anti-Neuronal Growth Factor Receptor (NGFR), p75, low affinity receptor. Detects a protein with a molecular weight of 75 kDa.
Formulation PBS (20mM), 0.14M NaCl, pH 7.3 containing 0.09%
sodium azide (NaN3) as preservative and 0.2% gelatin as stabilizer
Label: FITC
State: Liquid purified Ig fraction
Label: Fluorescein isothiocyanate isomer 1
Purification Affinity chromatography on Protein A
Conjugation FITC
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens nerve growth factor receptor (NGFR)
Synonyms TNFRSF16
Note This clone has also been reported to work in Immunohistochemistry (frozen and paraffin sections), Immunoprecipitation, Immunofluorescence, Functional assay (neutralization) and Western Blotting.

Protocol: Flow Cytometry Protocol:
1. Add 10 μl of antibody to 1 x 10e6 cells.
2. Incubate 30 minutes on ice in PBS containing 2-5% BSA.
3. Wash via centrifugation.
4. Analyze by flow cytometry.
Positive Control Cell Line: HS294T from ATCC

Procedure For General Staining Using Flow Cytometry: (For Non-Adherent Cells):
1. Add 0.3-1.0 μg anti-NGFR FITC or PE in 10 μl to one million cells in 100 μl PBS, 2% BSA.
2. Incubate on ice for 30 minutes.
3. Add 1.0 ml PBS, BSA and centrifuge for 5 minutes at 500xg to wash cells.
4. Suction off PBS, BSA and add 1.0 ml fresh PBS, BSA.
5. Analyze by flow cytometry.
6. HS294 T-cell line from ATCC can be used for positive control.
Reference Data
Protein Families Druggable Genome, Transmembrane
Protein Pathways Cytokine-cytokine receptor interaction, Neurotrophin signaling pathway
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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