CD95 (FAS) Mouse Monoclonal Antibody [Clone ID: UB2]

CAT#: AM26668RP-N

CD95 (FAS) mouse monoclonal antibody, clone UB2, PE

    • 50 Tests

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Product Data
Clone Name UB2
Applications FC
Recommended Dilution Flow cytomstry: 20 μl (ready for use).
For details see protocol below.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Recombinant human Fas
Specificity This antibody recognizes human Fas antigen.
Formulation PBS
Label: PE
State: Liquid Ig fraction
Stabilizer: 1% BSA
Preservative: 0.09% NaN3
Purification Affinity chromatography on protein A
Conjugation PE
Storage Store at 2-8 °C.
Stability Shelf life: one year from despatch.
Background It is now widely accepted that apoptosis plays an important role in the selection of immature thymocytes and Ag-primed peripheral T cells. Fas antigen is a cell-surface protein that mediates apoptosis. It is expressed in various tissues including the thymus and has structural homology to a number of cell-surface receptors, including tumor necrosis factor receptor and nerve growth factor receptor.
Synonyms FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6

This product was originally produced by MBL International.

Protocol: Flow cytometric analysis for floating cells
We usually use Fisher tubes or equivalents as reaction tubes for all steps described below.
1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and 0.1% NaN 3].
2) Resuspend the cells with washing buffer (5x10 6 cells/mL).
3) Add 50 μ L of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration.
4) Add 10 μ L of normal goat serum containing 1 mg/mL normal human IgG and 0.1% NaN 3 to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature.
5) Add 20 μ L of PE labeled anti-Fas monoclonal antibody (UB2). Mix well and incubate for 30 minutes at room temperature.
6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration.
7) Resuspend the cells with 500 μ L of the washing buffer and analyze by a flow cytometer.
(Positive control for Flow cytometry; transfectant)
Reference Data

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