CD95 (FAS) Mouse Monoclonal Antibody [Clone ID: UB2]

CAT#: AM26668FC-N

CD95 (FAS) mouse monoclonal antibody, clone UB2, FITC

Product images

Flow cytometric analysis of human Fas expression on transfectant. Open histogram indica tes the reaction of isotypic control to the cells . Shaded histogram indicates the reaction of AM26668FC-N to the cells.

Specifications

Product Data

• Clone Name: UB2
• Applications: FC
• Recommended Dilution: Flow cytometry: 20 μl (ready for use).
  For details see protocol below.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human Fas
• Specificity: This antibody recognizes the human Fas antigen specifically. Clone UB2 does not recognize the mouse Fas antigen.
• Formulation: BSA
  Label: FITC
  State: Liquid Ig fraction
  Stabilizer: 1% BSA
  Preservative:
  0.09% NaN₃
• Purification: Protein A agarose
• Conjugation: FITC
• Storage: Store at 2-8 °C.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 355 Human
  UniProt ID P25445
• Background: It is now widely accepted that apoptosis plays an important role in the selection of immature thymocytes and Ag-primed peripheral T cells. Fas antigen is a cell-surface protein that mediates apoptosis. It is expressed in various tissues including the thymus and has structural homology with a number of cell-surface receptors, includin g tumor necrosis factor receptor and nerve growth factor receptor.
• Synonyms: FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6
• Note: This product was originally produced by MBL International.
  
  Protocol: Flow cytometric analysis for floating cells
  We usually use Fisher tubes or equivalents as reaction tubes for all steps described below.
  1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and 0.1% NaN 3].
  2) Resuspend the cells with washing buffer (5 x 10 6 cells/mL).
  3) Add 50 μ L of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration.
  4) Add 20 μ L of Clear Back (human Fc receptor blocking reagent) to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature.
  5) Add 20 μ L of FITC labeled anti-Fas monoclonal antibody (UB2). Mix well and incubate for 30 minutes at room temperature.
  6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration.
  7) Resuspend the cells with 500 μ L of the washing buffer and analyze by a flow cytometer.
  (Positive control for Flow cytometry; transfectant)