CD95 (FAS) Mouse Monoclonal Antibody [Clone ID: UB2]
CD95 (FAS) mouse monoclonal antibody, clone UB2, FITC
Other products for "FAS"
Specifications
Product Data | |
Clone Name | UB2 |
Applications | FC |
Recommended Dilution | Flow cytometry: 20 μl (ready for use). For details see protocol below. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Recombinant human Fas |
Specificity | This antibody recognizes the human Fas antigen specifically. Clone UB2 does not recognize the mouse Fas antigen. |
Formulation | BSA Label: FITC State: Liquid Ig fraction Stabilizer: 1% BSA Preservative: 0.09% NaN3 |
Purification | Protein A agarose |
Conjugation | FITC |
Storage | Store at 2-8 °C. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Background | It is now widely accepted that apoptosis plays an important role in the selection of immature thymocytes and Ag-primed peripheral T cells. Fas antigen is a cell-surface protein that mediates apoptosis. It is expressed in various tissues including the thymus and has structural homology with a number of cell-surface receptors, includin g tumor necrosis factor receptor and nerve growth factor receptor. |
Synonyms | FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6 |
Note | This product was originally produced by MBL International. Protocol: Flow cytometric analysis for floating cells We usually use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and 0.1% NaN 3]. 2) Resuspend the cells with washing buffer (5 x 10 6 cells/mL). 3) Add 50 μ L of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration. 4) Add 20 μ L of Clear Back (human Fc receptor blocking reagent) to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature. 5) Add 20 μ L of FITC labeled anti-Fas monoclonal antibody (UB2). Mix well and incubate for 30 minutes at room temperature. 6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration. 7) Resuspend the cells with 500 μ L of the washing buffer and analyze by a flow cytometer. (Positive control for Flow cytometry; transfectant) |
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