Topoisomerase II alpha (TOP2A) (C-term) Mouse Monoclonal Antibody [Clone ID: 1C5]
Topoisomerase II alpha (TOP2A) (C-term) mouse monoclonal antibody, clone 1C5, Azide Free
|Applications||FC, IF, IHC, IP, WB|
|Recommended Dilution||Western blot: 0.1-0.5 µg/ml for chemiluminescence detection system.
Immunoprecipitation: 1-10 µg/200-300 µg/ml of cell extract.
Immunohoistochemistry on paraffin sections: Heat treatment is necessary; Microwave oven; 2 times for 10 minutes aech in 10 mM citrate buffer (pH 6.5).
Immunocytochemistry: 1-10 µg/ml.
Flow cytometry: 0.1-1 µg/ml (final contentration).
|Immunogen||Recombinant human Topoisomerase II alpha protein corresponding to C-termional 182 aa|
This antibody detects 170 kDa of human Topo II alpha on Western blot with total cell lysate from human cell lines.
|Formulation||PBS containing 50 % glycerol, pH 7.2. No preservative is contained.
State: Azide Free
State: Liquid Ig fraction
|Purification||Protein A agarose|
|Storage||Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.|
|Stability||Shelf life: One year from despatch.|
Topoisomerase II alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- and M-phases in transformed and developmentally regulated normal cells. Topoisomerase II alpha is also implicated in drug resistance of tumor cells.
|Synonyms||TOP2, Top-2, DNA topoisomerase 2-alpha, DNA topoisomerase II alpha isozyme|
This product was originally produced by MBL International.