Topoisomerase II alpha (TOP2A) (C-term) Mouse Monoclonal Antibody [Clone ID: 1C5]

CAT#: AM26595AF-N

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Topoisomerase II alpha (TOP2A) (C-term) mouse monoclonal antibody, clone 1C5, Azide Free



Size
    • 100 ug


Product images

Specifications

Product Data
Clone Name 1C5
Applications FC, IF, IHC, IP, WB
Recommended Dilution Western blot: 0.1-0.5 µg/ml for chemiluminescence detection system.
Immunoprecipitation: 1-10 µg/200-300 µg/ml of cell extract.
Immunohoistochemistry on paraffin sections: Heat treatment is necessary; Microwave oven; 2 times for 10 minutes aech in 10 mM citrate buffer (pH 6.5).
Immunocytochemistry: 1-10 µg/ml.
Flow cytometry: 0.1-1 µg/ml (final contentration).
Reactivity Human
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Recombinant human Topoisomerase II alpha protein corresponding to C-termional 182 aa
Specificity

This antibody detects 170 kDa of human Topo II alpha on Western blot with total cell lysate from human cell lines.

Formulation PBS containing 50 % glycerol, pH 7.2. No preservative is contained.
State: Azide Free
State: Liquid Ig fraction
Concentration lot specific
Purification Protein A agarose
Storage Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Stability Shelf life: One year from despatch.
Background
Topoisomerase II alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- and M-phases in transformed and developmentally regulated normal cells. Topoisomerase II alpha is also implicated in drug resistance of tumor cells.
Synonyms TOP2, Top-2, DNA topoisomerase 2-alpha, DNA topoisomerase II alpha isozyme
Note

This product was originally produced by MBL International.

Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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