Polymyxin B Mouse Monoclonal Antibody [Clone ID: 45]

CAT#: AM26366LE-N

Polymyxin B mouse monoclonal antibody, clone 45, Low Endotoxin

Specifications

Product Data

• Clone Name: 45
• Applications: ELISA, WB
• Recommended Dilution: Immunoassays.
  Western blot: The typical starting working dilution is 1/10.
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Specificity: The monoclonal antibody 45 reacts with Polymyxin B. It enables the possibilities to study quantitatively the interaction of PMB and LPS.
• Formulation: State: Low Endotoxin
  State: Liquid Culture Medium with a Low endotoxin Level
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Background: The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo.