GC1q R (C1QBP) Mouse Monoclonal Antibody [Clone ID: 74.5.2]

CAT#: AM26293PU-N

GC1q R (C1QBP) mouse monoclonal antibody, clone 74.5.2, Purified


USD 496.00

2 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Clone Name 74.5.2
Applications ELISA, FN, IF, IP, WB
Recommended Dilution Flow Cytometry: The typical starting working dilution is 1/50.
Functional Assays.
Immunoassays.
Immonofluorescence.
Immunoprecipitation.
Western blot: The typical starting working dilution is 1/50.
Reactivities Human, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Recombinant gC1qR corresponding to mature gC1qR (amino acids 74-282)
Specificity The monoclonal antibody 74.5.2 recognizes a cell membrane C1q binding molecule that recognises the globular heads of C1q. It is also present in plasma and the extracellular matrix. It is directed against epitopes in the XC15 peptide that contains a binding site for high-molecular-weight kininogen and Factor XII. Clone 74.5.2 recognizes both the mature (74-282) and truncated form, lacking residues 74-95.
Formulation PBS
State: Purified
State: Liquid 0.2 µm filtered Ig fraction
Stabilizer: 0.1% BSA
Concentration lot specific
Purification Protein G Chromatography
Conjugation Unconjugated
Storage Store undiluted at 2-8°C.
Stability Shelf life: one year from despatch.
Gene Name complement component 1, q subcomponent binding protein
Background

The molecule is an unusually acidic, single chain protein with an apparent molecular weight of 33 kDa. It does not possess a conventional sequence motif compatible with a membrane spanning segment nor a consensus site for a GPI anchor. gC1qR migrates as a single chain under both reducing and non-reducing conditions, but it behaves as an oligomer on gel-filtration in non-dissociating conditions. Its multimer formation may be a critical process by which the gC1qR molecule increases its affinity for multivalent ligands such as C1q.
gC1qR has been shown to inhibit complement activation by preventing the binding of C1q to antibodies, suggesting that the binding site for gC1qR and the binding site for immune complexes, which are present on the C1q globular ‘heads’, may be located at the same position. gC1qR is capable of interacting with several proteins involved in blood clotting, namely, thrombin, prothrombin, the heparinbinding form of vitronectin, the ternary complex, vitronectin-thrombin-antithrombin, as well as high-molecular-weight kininogen and Hageman factor. Besides its role in the complement pathway, gC1qR participates in enhancement of Fc-receptor and CR1-mediated phagocytosis, procoagulant activity on platelets, and chemotactic activity on mast cells, eosinophils, neutrophils, and fibroblasts.
gC1qR is expressed on a wide variety of cells and can serve as a binding site for plasma and microbial proteins. Its antigenic sites may be cryptic on cells in suspension but become exposed when the cells are fixed by bifunctional cross-linkers. Probably it is also expressed on the cell membrane as a tetramer. Crosslinking or activation may thus bring about a tetrameric assembly of gC1qR followed by a conformational change within the molecule, thereby exposing epitopes which are otherwise hidden. A form of gC1qR is also found inside the cell. Intracellular gC1qR has been shown to bind the cytoplasmic tail of the α1B-adrenergic receptor and to PKCµ.

Synonyms GC1QBP, HABP1, SF2P32, GC1q-R protein, p33, p32
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.