TLR2 Mouse Monoclonal Antibody [Clone ID: TL2.3]

CAT#: AM26265PU-N

TLR2 mouse monoclonal antibody, clone TL2.3, Purified

Specifications

Product Data

• Clone Name: TL2.3
• Applications: ELISA, FC, FN, IF, IHC, WB
• Recommended Dilution: Western blot: The typical starting working dilution is 1:50.
  Immunoassay.
  Flow cytometry: The typical starting working dilution is 1:50.
  Immunoflourescence.
  Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50.
  Functional assays: Can be used for stimulation of T cells.
• Reactivities: Canine, Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human TLR2-expressing CHO cells
• Specificity: The monoclonal antibody TL2.3 is specific for human TLR2 (CD282). TL2.3 is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human peripheral blood mononuclear cells.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
• Concentration: lot specific
• Purification: Protein G
• Conjugation: Unconjugated
• Storage:

  Store at 2 - 8 °C.
  

• Stability: Shelf life: one year from despatch.

• Gene Name: toll like receptor 2
• Database Link:
  Entrez Gene 7097 Human
  UniProt ID O60603
• Background: Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR2 has been identified as a receptor that is central to the innate immune response to lipoproteins of Gram-negative bacteria, several whole Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. A functional interaction between TLR2 and TLR6 in the cellular response to various bacterial products has been discovered. The currently accepted paradigm regards TLR2 as an essential receptor for many eubacterial cell wall components, including lipoproteins and peptidoglycan. Bacterial species as diverse as mycobacteria, spirochetes, mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2 (CD282).
• Synonyms: Toll-like receptor 2