CD20 (MS4A1) Mouse Monoclonal Antibody [Clone ID: CAT13.6E12]

CAT#: AM26056PU-N

CD20 (MS4A1) mouse monoclonal antibody, clone CAT13.6E12, Aff - Purified

Product Images

Immunohistochemistry on frozen sections: Staining of human tonsil with AM26056PU-N

Specifications

Product Data

• Clone Name: CAT13.6E12
• Applications: FC, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 0.2 μg/ml (1/1000).
  Has been described to work in FACS.
  Recommended Positive Control: Human tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody CAT13.6E12 recognizes the CD20 antigen.
  Antigen distribution on Isolated cells: The antibody stains 5-20% of human peripheral blood mononuclear cells in flow cytometry.
• Formulation: PBS, pH 7.2
  State: Aff - Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 5mg/ml BSA
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Restore by adding 0.5 ml distilled water
• Concentration: 0.2 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: membrane spanning 4-domains A1
• Database Link:
  Entrez Gene 931 Human
  UniProt ID P11836
• Background: CD20 belongs to the MS4A family (Membrane-spanning 4-domains subfamily A). Its expression is restricted to normal and neoplastic B cells. It is expressed by pre-B cells in the bone marrow, and expression persists during B cell maturation but is lost upon terminal differentiation into plasma cells.
  CD20 is not glycosylated. CD20 is not phosphorylated in resting B cells, but becomes heavily phosphorylated after mitogen stimulation. Three cell surface isoforms 33, 35 and 37kD, result from differential phosphorylation of a single core 33kD protein within the cytoplasmic domain. Multiple proteins are coimmunoprecipitated with CD20, suggesting that it may be a component of a multimeric surface receptor complex. Monoclonal antibodies against CD20 are used therapeutically, such as Rituximab (Mabthera) or Britumomab (Zevalin).
• Synonyms: MS4A1, Leu-16, Bp35, B-cell marker

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage