CD4 Mouse Monoclonal Antibody [Clone ID: EDU-2]

CAT#: AM26053PU-N

CD4 mouse monoclonal antibody, clone EDU-2, Aff - Purified

Product Images

Immunohistochemistry on frozen sections: Staining with AM26053PU-N on human tonsil

Specifications

Product Data

• Clone Name: EDU-2
• Applications: FC, IHC
• Recommended Dilution: Immunohistochemistry on frozen sections: 0.2 μg/ml (1:1000).
  Has been described to work in FACS.
  Suggested positive control: Human tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Stimulated human leukocytes
• Specificity: This antibody detects CD4.
• Formulation: Stock solution contains 0.2 mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5 mg/ml bovine serum albumin (BSA), and 0.09% sodium azide as a preservative.
  State: Aff - Purified
  State: Lyophilized Ig fraction
  Label: Reconstitute by adding 0.5ml distilled water.
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at 2-8°C. Following reconstitution store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens CD4 molecule (CD4), transcript variant 1
• Database Link:
  Entrez Gene 920 Human
  UniProt ID P01730
• Background: CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. It is mainly expressed by the T lymphocyte subset that recognizes antigens associated with self MHC class II molecules. CD4 is the primary receptor for HIV retroviruses. Like many cell surface markers, it is a member of the immunoglobulin superfamily.
• Synonyms: T-cell surface antigen T4/Leu-3
• Note: The antigen is CD4, a 55kD glycoprotein (reduced and nonreduced). The epitope has not been further characterized.
  Antigen distribution:
  Isolated cells: the antibody stains approximately 20-60% of
  human peripheral blood mononuclear cells in flow cytometry.

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane
• Protein Pathways: Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway