Cd4 Rat Monoclonal Antibody [Clone ID: GK1.5]
Cd4 rat monoclonal antibody, clone GK1.5, Low Endotoxin
|Applications||FC, FN, IF, IHC, IP|
|Recommended Dilution||Flow cytometry: 1 μg/million cells.
Immunoprecipitation: 1-2 μg/100-500 μg of protein in 1 ml lysate.
Immunohistochemistry (frozen sections).
Immunocytochemistry: 1-4 μg/ml.
Functional application: Isolation and depletion of CD4+ T cells, blocking of ligand binding to CD4.
|Immunogen||Mouse CTL clone V4 cells|
|Specificity||This antibody reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).|
|Formulation||Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized
State: Low Endotoxin
State: Liquid Ig fraction
|Purification||Protein-G affinity chromatography|
|Storage||Store undiluted at 2-8°C.
DO NOT FREEZE!
|Stability||Shelf life: one year from despatch.|
|Background||CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).|
|Synonyms||T-cell surface antigen T4/Leu-3|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.