Mouse Monoclonal Antibody [Clone ID: GFP. C2]
Mouse Monoclonal anti-GFP (+CFP) Antibody
Product Datasheet for 'AM20840PU-N'
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|Clone Name||GFP. C2|
|Recommend Dilution||ELISA, WB, Dot blot, IP|
|Immunogen||GFP from the Jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates.|
|Formulation||10mM PBS, PH 7.2|
|Purification||1-10 ng of purified GFP or GFP fusion proteins (Western blot developed with ECL)|
|Background||The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wild type GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.|