beta Actin (ACTB) Mouse Monoclonal Antibody [Clone ID: AC-15]

CAT#: AM20687PU-N

beta Actin (ACTB) mouse monoclonal antibody, clone AC-15, Purified

Product Images

Immunohistochemistry on paraffin Rat skeletal muscle sections

Specifications

Product Data

• Clone Name: AC-15
• Applications: IHC, IP, WB
• Recommended Dilution: Western Blot: 0.25-0.5 μg/ml.
  Immunohistochemistry on Paraffin Sections: 0.5-1 μg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Slightly modified beta-cytoplasmic actin N-terminal peptide,
  Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
• Specificity: This antibody reacts to Actin beta.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.02% Sodium Azide
• Reconstitution Method: Restore with 1.2% sodium acetate or neutral PBS.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 41.7 kDa
• Gene Name: actin, beta
• Database Link:
  Entrez Gene 11461 MouseEntrez Gene 81822 RatEntrez Gene 60 Human
  UniProt ID P60709
• Background: The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
• Synonyms: Actin cytoplasmic 1, Beta-Actin

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis