Hsp60 (HSPD1) Mouse Monoclonal Antibody [Clone ID: SJ-60]

CAT#: AM20630PU-N

Hsp60 (HSPD1) mouse monoclonal antibody, clone SJ-60, Purified

Product Images

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Specifications

Product Data

• Clone Name: SJ-60
• Applications: IHC, IP, WB
• Recommended Dilution: Western Blot: 2 - 4 μg/ml.
  Immunohistochemistry on paraffin sections: 4 - 8 μg/ml.
• Reactivities: Chicken, Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human heat shock protein 60 (HSP60)
• Specificity: This antibody reacts to HSP60.
• Formulation: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative
  State: Purified
  State: Lyphilized purified Ig fraction
• Reconstitution Method: Restore with 1.2% sodium acetate or neutral PBS
• Concentration: 0,1 mg/ml (after reconstitution with PBS)
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at -20°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: heat shock protein family D (Hsp60) member 1
• Database Link:
  Entrez Gene 63868 RatEntrez Gene 3329 Human
  UniProt ID P10809
• Background: Heat shock 60KD protein (HSP60) is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNFsecretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.
• Synonyms: HSP-60, HSPD1, Heat shock protein 60, Chaperonin 60, HuCHA60, GROEL, GroEL Homolog, CPN60

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: RNA degradation, Type I diabetes mellitus