FLT4 Mouse Monoclonal Antibody [Clone ID: 2E11]

CAT#: AM20379PU-N

FLT4 mouse monoclonal antibody, clone 2E11, Aff - Purified

Product Images

Western analysis of recombinant human soluble VEGFR3/FLT-4 using a Mouse anti-Human FLT-4 antibody.

• 

  Sandwich-ELISA using recombinant human soluble FLT-4 as standard ( DA3535). Mouse anti-Human FLT-4 Antibody Clone 2E11 was used as capture antibody, Biotinylated rabbit anti-Human FLT-4 was used for detection.

Specifications

Product Data

• Clone Name: 2E11
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 0.5-1.5 µg/ml, allows the detection of 0.25-0.5 ng/well rhVEGFR-3/FLT-4 in combination with compatible secondary reagents.
  Western Blot: 0.5-1 µg/ml with the appropriate secondary reagents to detect Human VEGFR-3/FLT-4. Depending on the visualisation method the detection limit for rh VEGFR-3/FLT-4 is approximately 5 ng/lane under reducing conditions.
  Immunofluorescence/Immunohistochemistry on Frozen Sections: 1-10 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human Vascular Endothelial Growth Factor Receptor 3 (rh VEGFR-3/FLT-4) extracellular domain.
• Specificity: This antibody detects Human VEGFR-3/FLT-4. Other species not tested.
• Formulation: PBS, pH 7.4 without preservatives
  State: Aff - Purified
  State: Lyophilized purified IgG fraction from Hybridoma Supernatant
• Reconstitution Method: Restore in Sterile water to a concentration of 0.1-1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: fms related tyrosine kinase 4
• Database Link:
  Entrez Gene 2324 Human
  UniProt ID P35916
• Background: VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk 1) and VEGFR-3 (FLT-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. These receptors play essential roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The VEGFR-3 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR-3 is composed of a 751 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGF R3 (Flt-4). The Flt-4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial a latter stage of development. It is important for lymphangiogenesis.
• Synonyms: VEGFR3, FLT4, VEGF Receptor 3

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Focal adhesion