IP10 (CXCL10) Mouse Monoclonal Antibody

CAT#: AM20277PU-N

IP10 (CXCL10) mouse monoclonal antibody, Azide Free

Product Images

Western Blot (Unreduced) using CXCL10 / IP-10 Antibody Cat.-No AM20277PU-N

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  Western Blot (Reduced) using CXCL10 / IP-10 Antibody Cat.-No AM20277PU-N

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  Sandwich ELISA using CXCL10 / IP-10 Antibody Cat.-No AM20277PU-N

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: In a Sandwich ELISA (assuming 100 μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human IP-10 when used with Biotinylated Antigen Affinity Purified anti-Human IP-10 (Cat.-No PP1039B1 or PP1039B2) as the detection antibody at a concentration of approximately 0.25 μg/ml.
  Western Blot: To detect Human IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml (non-reducing) or at a concentration of 1.0-2.0 μg/ml (reducing). When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IP-10 is 0.20-0.40 ng/lane under non-reducing conditions and 2.00-4.00 ng/lane under reducing conditions.
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Highly pure E.coli derived recombinant Human IP-10 (Cat.-No PA092)
• Specificity: Recognizes Human IP-10. Other species not tested.
• Formulation: PBS without preservatives
  State: Azide Free
  State: Lyophilized (sterile filtered) purified IgG fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: C-X-C motif chemokine ligand 10
• Database Link:
  Entrez Gene 3627 Human
  UniProt ID P02778
• Background: Chemokines are members of a superfamily of inducible, secreted, proinflammatory cytokines. Members of the chemokine family exhibit 20% to 50% homology in their predicted amino acid sequences and are divided into several subfamilies. The C-X-C chemokine subfamily includes IL-8, GROa/b/g (and the murine homologs KC, MIP-2a and MIP-2b), platelet basic protein, ENA-78, GCP-2, PF4, IP-10 (and its murine homolog, CRG) and MIG.
• Synonyms: C-X-C motif chemokine 10, Small-inducible cytokine B10, Gamma-IP10, INP10, SCYB10

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway