Heparan-Sulfate-Proteoglycan Mouse Monoclonal Antibody [Clone ID: 2E2/B5]

CAT#: AM20231PU-N

Heparan-Sulfate-Proteoglycan mouse monoclonal antibody, clone 2E2/B5, Purified

Specifications

Product Data

• Clone Name: 2E2/B5
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA (less than 1 µg/ml).
  Western blot (1 µg/ml).
  Immunohistochemistry (1 µg/ml)
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified Human small basement membrane Heparan Sulfate Proteoglycan (HS-PG)
• Specificity: This Monoclonal antibody is specific for a core protein epitope of a Human small basement membrane Heparan Sulfate Proteoglycan (HS-PG).
  AM20231PU recognizes an epitope different from that recognized by clones 1F10/B8 (Cat.No AM02102PU) and 2H7/G11 (Cat.-No AM02103PU).
• Formulation: 50 mM TRIS pH 7.4
  State: Purified
  State: Lyophilized purifed IgG fraction from Cell Culture Supernatant
• Reconstitution Method: Restore in aqua bidest to 1 mg/ml
• Purification: Protein G Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: The principal molecular structure of basement membranes has been elucidated during the past two decades and it was shown that for example in the glomerular basement membrane (GBM) HS-PG are responsible for the selective filtration process. Removal of HS-PG will lead to proteinuria. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that (at least two) other basement membrane HS-PG do exist: Agrin, originally discovered as an important component of the neuromuscular junction and a novel small HS-PG, that was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200 kDa (aorta) and 30-160 kDa (kidney) and a core protein size of 24 kDa or 22 kDa, respectively, was localized by immunohistoche-mistry to the basement membrane. Amino acid sequence ana-lysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin.
• Synonyms: small Heparan sulfate proteoglycan core protein