Phospholipase C gamma 1 (PLCG1) (970-990) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2D5]

CAT#: AM20220PU-N

Phospholipase C gamma 1 (PLCG1) (970-990) (incl. pos. control) mouse monoclonal antibody, clone 2D5, Purified

Product Images

Detection of endogenous Phospholipase C gamma: Whole cell lysates of EGF stimulated serum starved tumor cells (ca. 20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab AM20220PU-N PLCG antibody (Clone 2D5) at 0.5 ug/ml for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: A549 Lane 3: SKOV3 Lane 4: OVCAR5 Lane 5: HaCaT Lane 6: PC3 Lane 7: HeLa Lane 8: HepG2

Specifications

Product Data

• Clone Name: 2D5
• Applications: WB
• Recommended Dilution: Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L.
  Included Positive Control: Cell lysate from untreated SKOV-3 cells (See Protocols for more details).
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide conjugated to hemocyanin corresponding to amino acids 970-990 of PLCgamma (Phospholipase C gamma).
• Specificity: This monoclonal PLCG antibody (Clone 2D5) specifically recognizes Phospholipase C gamma at 150 kDa in Western blot applications.
• Formulation: PBS
  State: Purified
  State: Lyophilized purified Ig fraction.
  Stabilizer: PEG and Sucrose
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.
• Conjugation: Unconjugated
• Storage: Store lyophilized at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20 to -80°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 150 kDa
• Gene Name: phospholipase C gamma 1
• Database Link:
  Entrez Gene 18803 MouseEntrez Gene 25738 RatEntrez Gene 5335 Human
  UniProt ID P19174
• Background: Phospholipase C gamma (PLCG) binds to receptor tyrosine kinases, e.g. EGFR, PDGFR, leading to phosphorylation of PLCgamma at Tyr 771, 783 and 1245. Phosphorylation of Tyr 783 leads to enzymatic activation of PLCgamma. PLC-gamma hydrolyzes phosphatidylinostitol-4,5-bisphosphate (PIP2), thus generating the second messengers inostitol-1,4,5-triphosphate (IP3) and diacylglycerol (DAG).
• Synonyms: Phospholipase C gamma, PLC-gamma
• Note: Protocol: Positive Control Provided: Cell lysate from untreated SKOV-3 cells
  
  Description: Cell lysate from untreated SKOV-3 cells, ovary adenocarcinoma (Human)
  
  Format: Lyophilized cell lysate from serum starved SKOV-3 cells.
  
  Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Storage: Aliquote and store frozen.
  Avoid repeated freeze/thaw cycles.
  
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
  Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glioma, Inositol phosphate metabolism, Leukocyte transendothelial migration, Metabolic pathways, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, T cell receptor signaling pathway, VEGF signaling pathway, Vibrio cholerae infection