Phospholipase C gamma 1 (PLCG1) (970-990) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2D5]

CAT#: AM20220PU-N

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Phospholipase C gamma 1 (PLCG1) (970-990) (incl. pos. control) mouse monoclonal antibody, clone 2D5, Purified



USD 512.00


Availability*
2 Weeks

Size
    • 100 ug


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Specifications

Product Data
Clone Name 2D5
Applications WB
Recommended Dilution Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L.
Included Positive Control: Cell lysate from untreated SKOV-3 cells (See Protocols for more details).
Reactivities Canine, Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Synthetic peptide conjugated to hemocyanin corresponding to amino acids 970-990 of PLCgamma (Phospholipase C gamma).
Specificity This monoclonal PLCG antibody (Clone 2D5) specifically recognizes Phospholipase C gamma at 150 kDa in Western blot applications.
Formulation PBS
State: Purified
State: Lyophilized purified Ig fraction.
Stabilizer: PEG and Sucrose
Preservative: 0.09% Sodium Azide
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation Unconjugated
Storage Store lyophilized at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20 to -80°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Predicted Protein Size 150 kDa
Gene Name Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant 2
Background Phospholipase C gamma (PLCG) binds to receptor tyrosine kinases, e.g. EGFR, PDGFR, leading to phosphorylation of PLCgamma at Tyr 771, 783 and 1245. Phosphorylation of Tyr 783 leads to enzymatic activation of PLCgamma. PLC-gamma hydrolyzes phosphatidylinostitol-4,5-bisphosphate (PIP2), thus generating the second messengers inostitol-1,4,5-triphosphate (IP3) and diacylglycerol (DAG).
Synonyms Phospholipase C gamma, PLC-gamma
Note Protocol: Positive Control Provided: Cell lysate from untreated SKOV-3 cells

Description: Cell lysate from untreated SKOV-3 cells, ovary adenocarcinoma (Human)

Format: Lyophilized cell lysate from serum starved SKOV-3 cells.

Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Storage: Aliquote and store frozen.
Avoid repeated freeze/thaw cycles.

Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
Protein Families Druggable Genome
Protein Pathways Calcium signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glioma, Inositol phosphate metabolism, Leukocyte transendothelial migration, Metabolic pathways, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, T cell receptor signaling pathway, VEGF signaling pathway, Vibrio cholerae infection
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