GFP Mouse Monoclonal Antibody [Clone ID: 168AT1211]

CAT#: AM11009PU-N

GFP mouse monoclonal antibody, clone 168AT1211, Purified


USD 599.00

2 Weeks*

Size
    • 200 ul

Product Images

Specifications

Product Data
Clone Name 168AT1211
Applications IF, IHC, WB
Recommended Dilution Western blotting: 1/4,000.
Immunohistochemistry: 1/50-1/100.
Immunofluorescence: 1/50-1/100.
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Purified His-tagged GFP protein was used to produced this monoclonal antibody.
Formulation PBS containing 0.09% (W/V) Sodium Azide as preservative
State: Purified
State: Liquid purified Ig fraction
Concentration lot specific
Purification Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Conjugation Unconjugated
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Predicted Protein Size 27 kDa
Background Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with asecondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunoprecipitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. This monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.
Synonyms Green fluorescent protein, GFP-Tag
Reference Data

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.