Digoxigenin Mouse Monoclonal Antibody [Clone ID: 21H8]

CAT#: AM10164AP-N

Digoxigenin mouse monoclonal antibody, clone 21H8, AP

Specifications

Product Data

• Clone Name: 21H8
• Applications: ELISA, IHC, ISH, SB, WB
• Recommended Dilution:

  ELISA: 1/500-1/5000.
  Southern Blot: 1/500-1/5000.
  Western Blot: 1/500-1/5000.
  In Situ Hybridization.
  Immunohistochemistry: 1/200-1/2000.

• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Digoxigenin
• Specificity: Reacts with free and bound Digoxigenin.
• Formulation: 10mM Tris, 0.14M NaCl, 1mM MgCl₂/ZnCl₂
  Label: AP
  State: Liquid purified IgG fraction
  Stabilizer: 50% Glycerol
  Preservative: 0.02% Thiomersal
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: AP
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background:

  Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.