Collagen V (COL5A1) Mouse Monoclonal Antibody [Clone ID: 18G5]
Collagen V (COL5A1) mouse monoclonal antibody, clone 18G5, Purified
|Applications||ELISA, IF, IHC, IP, WB|
Western Blot: 1/200-1/1000.
Immunohistochemistry on Paraffin Sections: 15 µg/ml.
|Immunogen||Purified recombinant N alpha 1 fragment: Human N alpha 1 (V).
The epitope is located in the COL2-NC2 region.
|Specificity||Reacts with Human pro alpha 1(V) chain.|
State: Lyophilized powder
|Reconstitution Method||Restore in distilled water.|
|Purification||Affinity Chromatography on Protein A|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Gene Name||collagen type V alpha 1|
|Background||Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure.Type V Collagen is a member of group I Collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V Collagen binds to DNA, heparan sulfate, Thrombospondin, Heparin, and Insulin. Collagen V is composed of several subunits as follows: trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Type V Collagen is closely related to type XI Collagen and it is possible that the Collagen chains of types V and XI constitute a single collagen type with tissue specific chain combinations. Mutations in this gene are associated with Ehlers Danlos syndrome, types I and II.|