Glypican 3 (GPC3) Mouse Monoclonal Antibody [Clone ID: 1G12]

CAT#: AM10091SU-N

Glypican 3 (GPC3) mouse monoclonal antibody, clone 1G12, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human hepatocellular carcinoma stained with Glypican-3 antibody Cat.-No. AM10091SU-N using peroxidase conjugate and DAB chromogen. Note membranous and granular cytoplasmic staining of tumor cells.

Specifications

Product Data

• Clone Name: 1G12
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/150
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Hepatocellular carcinoma.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A fragment containing the last 70 amino acids of the core glypican-3.
• Specificity: The 1G12 monoclonal antibody has been used to assess GPC3 expression in malignant and nonmalignant liver tissue samples by Immunohistochemistry in formalin-faxed and paraffin-embedded tissue.
  Cellular Localization: Cytoplasmic and membrane.
• Formulation: State: Supernatant
  State: Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: glypican 3
• Database Link:
  Entrez Gene 3958 HumanEntrez Gene 2719 Human
  UniProt ID P51654
• Background: Glypican-3 (GPC3) is a member of the glypican family of glycosyl phosphatidylinositol-anchored cell-surface heparan sulfate proteoglycans. Studies have shown that GPC3 is expressed at the protein level in most hepatocellular carcinomas, but it is undetectable in normal liver and benign hepatic lesions, including dysplastic and cirrhotic nodules. In addition, GPC3 is significantly elevated in the serum of a large proportion of patients with hepatocellular carcinoma. Based on these results, it has been proposed that GPC3 could be a useful marker to differentiate between benign and malignant liver diseases.
• Synonyms: Intestinal protein OCI-5, GTR2-2, MXR7, OCI5

Reference Data

• Protein Families: Secreted Protein