PCNA Mouse Monoclonal Antibody [Clone ID: PC10]

CAT#: AM10035FC-N

PCNA mouse monoclonal antibody, clone PC10, FITC

Product Images

Staining of KM-H2 cells (permeabilised) with MOUSE ANTI PCNA:FITC ([SM1421F])

Specifications

Product Data

• Clone Name: PC10
• Applications: FC, IF
• Recommended Dilution: Flow Cytometry: Use 10 µl of Neat-1/10 of dilutued antibnody to label 10⁶ cells in 100 µl. Cell permeabilisation is required for this application.
• Reactivities: Amphibian, Drosophila, Fish, Mammalian, Yeast
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Rat PCNA made in the protein A expression vector pR1T2T.
• Specificity: This antibody recognises the proliferating cell nuclear antigen (PCNA), a nuclear protein vital for cellular DNA synthesis.
  PCNA is highly conserved between mammalian species and other vertebrates. Mouse anti Human PCNA, clone PC10 has been used for the detection of PCNA in a number of species including Rat (Elsässer et al. 1994), Mouse (Park et al. 2008), Chicken (Franz-Odendaal 2008) and Abalone (Harris et al. 2006).
• Formulation: PBS
  Label: FITC
  State: Liquid purified IgG fraction
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: proliferating cell nuclear antigen
• Database Link:
  UniProt ID P12004
• Background: Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase delta in eukaryotic cells. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. PCNA was originally identified as an antigen that is expressed in the nuclei of cells during the DNA synthesis phase of the cell cycle It is increased during late G1 phase and S phase of the cell cycle and declines during G2 and M phases.
• Synonyms: Cyclin