TUBB2B Mouse Monoclonal Antibody [Clone ID: AT5B2]

CAT#: AM09384PU-N

TUBB2B mouse monoclonal antibody, clone AT5B2, Purified

Product Images

Western blot analysis: The Cell lysate (40ug) and Mouse tissue extracts (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin (1/5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: Jurkat cell lysate. Lane 2: A549 cell lysate. Lane 3: A431 cell lysate. Lane 4: HepG2 cell lysate. Lane 5: Mouse Brain tissue extract.

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  Immunofluorescence of A431 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1/500) with Alexa Fluor 488 (Green).

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  Immunofluorescence of Balb/3T3 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1/200) with Alexa Fluor 488 (Green).

Specifications

Product Data

• Clone Name: AT5B2
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western blot:1/1000-1/10000.
  Immunofluorescence: 1/1000-1/5000.
  Flow Cytometry: 1/100-1/1000.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human beta-Tubulin TUBB2B (1-445aa) purified from E. coli
• Specificity: The antibody recognizes Human and Mouse beta-Tubulin. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: tubulin beta 2B class IIb
• Database Link:
  Entrez Gene 73710 MouseEntrez Gene 347733 Human
  UniProt ID Q9BVA1
• Background: Tubulin is a major cytoskeleton component that has five distinct forms. Multiple beta Tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
• Synonyms: Tubulin beta-2B chain, beta II Tubulin B, beta-Tubulin, RP11-506K6.1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection