Sumo 2 (SUMO2) Mouse Monoclonal Antibody [Clone ID: AT10F1]

CAT#: AM09370PU-N

Sumo 2 (SUMO2) mouse monoclonal antibody, clone AT10F1, Purified

Product Images

Cell lysates of HeLa (35ug) and recombinant proteins (10ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human SUMO2/3 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

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  Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human SUMO2/3 antibody (1:500) with Texas Red (Red).

Specifications

Product Data

• Clone Name: AT10F1
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (~1/1000).
  Immunofluorescence (1/250-1/500).
  Immunohistochemistry on Paraffin embedded tissues (1/50).
  Flow Cytometry (~5 µg in 10⁶ cells).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human SUMO2 (1-93aa) purified from E. coli
• Specificity: The antibody recognizes Human SUMO2/3. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: small ubiquitin-like modifier 2
• Database Link:
  Entrez Gene 6613 Human
  UniProt ID P61956
• Background: SUMO (small ubiquitin-related modifier) is the best-characterized member of a growing family of ubiquitin-related proteins. 8–11 kDa that covalently modify various intracellular proteins. It resembles ubiquitin in its structure, its ability to be ligated to other proteins. SUMO regulates cellular function of a variety of target proteins. SUMO proteins are expressed as their precursor forms. Cleavage of the residues after the ‘GG’ region of these precursors by SUMO-specific proteases in maturation is a prerequisite for subsequent sumoylation. Notably, SUMO2 and SUMO3 precursors have 96% sequence identity, and recent studies have shown protein substrates conjugated with SUMO2 or SUMO3 have similar, if not identical, biological consequences.
• Synonyms: SUMO-2, SMT3B, SMT3H2, HSMT3, Sentrin-2, SUMO-3, SMT3 homolog 1, SMT3H1

Reference Data

• Protein Families: Druggable Genome