CLEC4E Mouse Monoclonal Antibody [Clone ID: AT16E3]

CAT#: AM09361PU-S

CLEC4E mouse monoclonal antibody, clone AT16E3, Purified

Product Images

Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MINCLE (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Paraffin embedded sections of colorectal cancer tissue were incubated with anti-Human MINCLE antibody (1/50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

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  Immunofluorescence of human HeLa cells stained with monoclonal anti-human MINCLE antibody (1/500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).

Specifications

Product Data

• Clone Name: AT16E3
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western blot: 1/250-1/1000.
  Immunofluorescence: 1/250-1/500.
  Immunohistochemistry on Paraffin Embedded tissues: 1/50.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant Human MINCLE (41-219aa) purified from E. coli
• Specificity: The antibody recognizes Human CLEC4E (MINCLE). Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens C-type lectin domain family 4 member E (CLEC4E)
• Database Link:
  Entrez Gene 26253 Human
  UniProt ID Q9ULY5
• Background: MINCLE (macrophage inducible C-type lectin, also called CLEC4E or CLECSF9), which is a diverse family of protein which was originally defined by their ability to recognize a wide range of ligand of carbohydrate structure. MINCLE expressed in macrophages subjected to several types of stress. It plays an essential role in response to trehalose-6,6'-dimycolate (TDM) and activated by a synthetic analogue, trehalose dibehenate (TDB). Recently it was reported that MINCLE is associated with an immunoreceptor tyrosine-based activation motif–containing Fc receptor γ chain (FcRγ) and functions as an activating receptor for damaged self- and non–self-pathogenic fungi.
• Synonyms: CLECSF9, MINCLE

Reference Data

• Protein Families: Druggable Genome, Transmembrane