Cytokeratin 18 (KRT18) (Multi-Epitope Cocktail) Mouse Monoclonal Antibody [Clone ID: Ks18.04/18.27/18.5/9B1/18.174]

CAT#: AM09125SU-N

Cytokeratin 18 (KRT18) (Multi-Epitope Cocktail) mouse monoclonal antibody, clone Ks18.04/18.27/18.5/9B1/18.174, Supernatant

Specifications

Product Data

• Clone Name: Ks18.04/18.27/18.5/9B1/18.174
• Applications: IHC, WB
• Recommended Dilution: Immunoblotting (Western).
  Immunohistochemistry on Frozen and paraffin-Embedded Tissue (only when using the microwave method) and Cytological Material.
  Working Dilution: Ready-to-use for Immunohistochemistry.
  Incubation Time: 1h at RT, extended with paraffin sections (over night at 2-8°C).
• Reactivities: Bovine, Canine, Fish, Hamster, Human, Mammalian, Mouse, Porcine, Rat, Sheep
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Keratin K18 of Human and Bovine origin.
• Specificity: The antibody cocktail represents an excellent marker to discriminate simple epithelia from those of different origin.
  Tumors Specifically Detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma.
  Polypeptide Reacting: Mr 45 000 polypeptide (human and bovine K18; formerly
  also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
  Reactivities on Cultured Cell Lines: HeLa and MCF-7.
• Formulation: State: Supernatant
  State: Hybridoma Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens keratin 18, type I (KRT18), transcript variant 1
• Database Link:
  Entrez Gene 16668 MouseEntrez Gene 294853 RatEntrez Gene 3875 Human
  UniProt ID P05783
• Background: Cytokeratin 18 is an acidic keratin which is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas express only Cytokeratins 8 and 18.
• Synonyms: Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18

Reference Data

• Protein Pathways: Pathogenic Escherichia coli infection