Cofilin 1 (CFL1) Mouse Monoclonal Antibody [Clone ID: AT1C1]
Specifications
| Product Data | |
| Clone Name | AT1C1 |
|---|---|
| Application | ELISA, ICC/IF, IHC, WB |
| Recommended Dilution | ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (10 µg/ml). This Cofilin 1 antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1, kappa |
| Clonality | Monoclonal |
| Immunogen | Recombinant human Cofilin 1 (1-166aa) purified from E. coli |
| Specificity | The antibody recognizes Human Cofilin-1. Other species not tested. |
| Buffer | Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol |
| Concentration | 1mg/ml (determined by BCA assay) |
| Purification | By protein-G affinity chromatography |
| Conjugation | Unconjugated |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | cofilin 1 |
| Database Link | |
| Background | Cofilin is a family of actin-binding proteins which disassembles actin filaments. It is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. |
| Synonyms | 18 kDa phosphoprotein(p18), CFL, CFL1, Cofilin 1, Cofilin 1 non muscle, Cofilin non muscle isoform, P18 |
| Reference Data | |
| Protein Categories | Cytokines, Intracellular Proteins |
| Protein Families | Druggable Genome |
| Protein Pathways | Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton |
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