NANOG (1-154) Mouse Monoclonal Antibody [Clone ID: 5A10]
Specifications
| Product Data | |
| Clone Name | 5A10 |
|---|---|
| Application | ELISA, FACS, FC, IHC, WB |
| Recommended Dilution | ELISA. Western blot (1/500-1/2,000). Flow Cytometry. Immunohistochemistry on Paraffin Sections (1/50-1/100). |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG2a, kappa |
| Clonality | Monoclonal |
| Immunogen | Recombinant human Nanog (1-154aa) purified from E. coli |
| Specificity | The antibody recognizes NANOG. |
| Buffer | Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol |
| Concentration | 1mg/ml (determined by BCA assay) |
| Purification | By protein-G affinity chromatography |
| Conjugation | Unconjugated |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | Nanog homeobox |
| Database Link | |
| Background | Nanog is a multidomain homeobox transcription factor that functions to maintain the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. When Nanog expression is downregulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53. |
| Synonyms | Nanog homeobox, hNanog, Homeobox protein NANOG, Homeobox transcription factor Nanog |
| Reference Data | |
| Protein Categories | Intracellular Proteins, Transciption Factors |
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