Nuclei Mouse Monoclonal Antibody [Clone ID: 235-1]

CAT#: AM08257SU-N

Nuclei mouse monoclonal antibody, clone 235-1, Supernatant

Product Images

Figure 1. Immunostaining of HeLa cells showing specific labeling of their nuclei using anti-Nuclei Antibody Cat.-No AM08257SU-N (Clone 235-1).

Specifications

Product Data

• Clone Name: 235-1
• Applications: IF, IHC, IP
• Recommended Dilution: Immunoprecipitation.
  Immunofluorescence: 1/100.
  Immunohistochemitry: 1/100.
  The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. Also works for Immunohistochemistry on paraformaldehyde fixed cryostat sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human cell homogenate.
• Specificity: This antibody is an excellent Marker for Human cells in xenographic model research. It reacts specifically with Human cells, including neurons and embryonic stem cells, with no reactivity to Rat or Mouse cells.
  Stains nuclei of all human cell types giving a diffuse nuclear staining pattern.
  Chromosomes are negatively stained in metaphase cells.
  By Immunoprecipitation the antibody reacts with an 80 kDa and 70 kDa band.
• Formulation: PBS
  State: Supernatant
  State: Liquid Culture Supernatant.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.