Nuclei (Nuclear Marker) Mouse Monoclonal Antibody [Clone ID: 235-1]

SKU
AM08257PU-S
Nuclei (Nuclear Marker) mouse monoclonal antibody, clone 235-1, Purified
  $665.00
2 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Clone Name 235-1
Application FC, IF, IHC, IP
Recommended Dilution Flow Cytometry: 1-2ug/million cells
Immunofluorescence: 1-2ug/ml
Immunoprecipitation: 1-2ug/500ug protein lysate
Immunocytochemistry (Acetone-fixed cells): 1-2ug/ml for 30 minutes at RT
Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT
Optimal dilution for any specific application should be determined.
Recommended Positive Control: Tonsil
Reactivity Human, Primate
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Nuclei of human myeloid leukemia biopsy cells.
Specificity This Monoclonal Antibody is part of a new panel of rea gents, which recognizes subcellular organelles or compartments of Human cells.
These marker s may be useful in identification of these organelles in cells, tissues, and biochemica l preparations. Monoclonal Antibody 235-1 recognizes an antigen associated with the nuclei in Human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear ma rker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Cellular Localization: Nuclei.
Negative Species: Mouse, Rat, Chicken.
Buffer 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Predicted Protein Size 70 kda and 80 kDa
Background Hepatoblastoma is the most common primary tumor of the liver in children. The use of specific hepatocyte markers and also of alpha Fetoprotein or carcinoembryonic antigen are useful for the identification of normal and malignant fetal hepatocytes.
Synonyms Human Nuclear Antigen
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.