NOP1 Mouse Monoclonal Antibody [Clone ID: 38F3]

CAT#: AM08249PU-N

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NOP1 mouse monoclonal antibody, clone 38F3, Ig Fraction


Product Datasheet for 'AM08249PU-N'


USD 365.00


Availability*
3 Days

Size
    • 100 ul


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Specifications

Product Data
Clone Name 38F3
Applications IF, IHC, WB
Recommended Dilution Western Blot: 1/1,000.
Immunofluorescence: 1/500.
Immunohistochemistry.
Reactivity C. elegans, Drosophila, Human, Plant, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Yeast nuclear preparations.
Specificity Specific for the ~34kDa Fibrillarin /Nop1p protein.
This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).
Formulation State: Ig Fraction
State: Liquid Total IgG fraction
Preservative: 10 mM Sodium Azide
Storage Upon receipt, store (in aliquots) at -20°C to -80°C.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3).
Synonyms FIB1, FLRN, Fibrillarin, RNU3IP1, NOP1, LOT3, Nucleolar protein 1, Nucleolar Marker
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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