MMP9 Mouse Monoclonal Antibody [Clone ID: SB15c]

CAT#: AM08200PU-N

MMP9 mouse monoclonal antibody, clone SB15c, Purified

Product Images

Immunohistochemistry (Paraffin-Embedded Sections): Human colon tissue stained with at 5 ug/ml.

Specifications

Product Data

• Clone Name: SB15c
• Applications: ELISA, IHC
• Recommended Dilution: ELISA: ≤ 1 µg/ml.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry-Paraffin Sections (Ref-1-3).
  Immunoblotting (Reported in literature, See also Ref.4).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant full length MMP-9.
• Specificity: This antibody is specific for Human MMP-9.
  Does not cross react to Human MMP-1, MMP-2 or MMP-3.
• Formulation: 100 mM Borate buffered saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: matrix metallopeptidase 9
• Database Link:
  Entrez Gene 4318 Human
  UniProt ID P14780
• Background: MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III and is produced by normal alveolar macrophages and granulocytes. The expression of MMP9 increases in Epstein-Barr virus infected lymphoma derived cell lines and may be of significance in typically invasive nasopharyngeal carcinomas. MMP9 is constitutively produced by some tumor cell lines (e.g.: HT1080, HL60, U937) but not by most quiescent cells and tissues. Treatment of cells with the phorbol ester TPA stimulates production of MMP9 in some cell types, but the low protein levels produced (pg/ml) often require concentration of cell culture media to visualize the bands by Western blotting.
• Synonyms: Matrix metalloproteinase-9, MMP9, 92 kDa type IV collagenase, 92 kDa gelatinase, GELB, Gelatinase B, CLG4B

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Bladder cancer, Leukocyte transendothelial migration, Pathways in cancer