FADD Mouse Monoclonal Antibody [Clone ID: 1A11]

CAT#: AM08189SE-N

FADD mouse monoclonal antibody, clone 1A11, Sepharose

Specifications

Product Data

• Clone Name: 1A11
• Applications: ELISA, IP, WB
• Recommended Dilution: ELISA.
  Immunoprecipitation.
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody is specific for FADD.
• Formulation: 2 ml of 100 mM Borate buffered saline, pH 8.2.
  No preservatives or amine-containing buffer salts added
  Label: Sepharose
  State: Liquid purified Ig fraction.
  Label: 4B (SEPH)
• Conjugation: Sepharose
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Store protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Fas associated via death domain
• Database Link:
  Entrez Gene 8772 Human
  UniProt ID Q13158
• Background: Fas-associated death domain (FADD, also known as MORT1) is an important apoptosis adapter molecule involved in the Fas-mediated cell death pathway. FADD associates with the intracellular domain of FAS (CD95/APO-1) through their respective C-terminal death domains (DD). The N-terminal region of FADD contains a death effectors domain (DED) which recruits and binds cytosolic proteins such as FLICE/caspase-8. The subsequent activation of FLICE by proteolytic cleavage leads to the activation of the caspase-mediated (ICE-related proteases) apoptotic cascade. Overexpression of FADD induces apoptosis which is inhibited by CrmA, a specific inhibitor of the interleukin-1a-converting enzyme. FADD has also been shown under certain circumstances to promote cell survival and proliferation. (Ref.1-6)
• Synonyms: FAS-associated death domain protein, MORT1, GIG3
• Note: Shake well before use.