Bu-1b Mouse Monoclonal Antibody [Clone ID: 5-11G2]

CAT#: AM08135PU-N

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Bu-1b mouse monoclonal antibody, clone 5-11G2, Purified



USD 442.00


Availability*
3 Days

Size
    • 500 ug


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Specifications

Product Data
Clone Name 5-11G2
Applications FC, IHC, IP
Recommended Dilution Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1)
Immunoprecipitation. (Ref.1)
Immunohistochemistry on Frozen Sections. (Ref.3)
Reactivities Chicken
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Bursal cells from 2- to 5-week-old CHA (Bu-1b) strain Chickens.
Specificity This antibody recognizes Chicken Bu-1b alloantigen (Mr. 70 kDa). It reacts with lymphoid cells from CHA and H.B14Ab strains but does not react with cells from H.B14Aa.
Formulation 100 mM Borate Buffered Saline, pH 8.2.
No preservatives or amine-containing buffer salts added.
State: Purified
State: Liquid purified Ig fraction.
Concentration lot specific
Conjugation Unconjugated
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Bu1b is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has no recognizable similarity to known mammalian molecules and therefore represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. Chicken Bu1b, a product of the Bu1b allele, is expressed on the cell surface as a disulfide linked homodimer. In 10 week old H.B15 and H.B2 chickens, Bu1 is found on 85 to 90% of bursal cells, 2 to 8% of thymocytes, 15 to 27% of spleen cells, and 2 to 18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu1 alleles (Bu1 a/b), Bu1a does not exhibit allelic exclusion.
Synonyms B6.2, chicken B-cell marker
Reference Data
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