Bu-1b Mouse Monoclonal Antibody [Clone ID: 5-11G2]
$525.00
3 Days*
Specifications
| Product Data | |
| Clone Name | 5-11G2 |
|---|---|
| Application | FC, IHC, IP |
| Recommended Dilution | Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1) Immunoprecipitation. (Ref.1) Immunohistochemistry on Frozen Sections. (Ref.3) |
| Reactivity | Chicken |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Bursal cells from 2- to 5-week-old CHA (Bu-1b) strain Chickens. |
| Specificity | This antibody recognizes Chicken Bu-1b alloantigen (Mr. 70 kDa). It reacts with lymphoid cells from CHA and H.B14Ab strains but does not react with cells from H.B14Aa. |
| Buffer | 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction. |
| Concentration | lot specific |
| Conjugation | Unconjugated |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Database Link | |
| Background | Bu1b is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has no recognizable similarity to known mammalian molecules and therefore represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. Chicken Bu1b, a product of the Bu1b allele, is expressed on the cell surface as a disulfide linked homodimer. In 10 week old H.B15 and H.B2 chickens, Bu1 is found on 85 to 90% of bursal cells, 2 to 8% of thymocytes, 15 to 27% of spleen cells, and 2 to 18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu1 alleles (Bu1 a/b), Bu1a does not exhibit allelic exclusion. |
| Synonyms | B6.2, chicken B-cell marker |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.